Capillary Electrophoresis(CE)

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Abstract

Capillary Electrophoresis(CE) is one of separation analyses for ions in an aqueous solution.
Ion-chromatography can not be applied to qualitative and quantitative analysis for organic acids like acrylic acid etc. though, CE is applicable to those organic acids.

Principle and Characteristics

Principle
A sample (minimal volume of a sample solution is 1 to 50nL) is introduced to a fused silica capillary tube (a internal diameter ≦ 100 μm) and then, high voltage is applied to the both sides of the capillary tube.
A buffer solution including a sample in a capillary starts to flow(Electro Osmotic Flow:EOF) to a UV detector.
Ions are separated based on a different electrophoretic mobility of each ion.

Characteristics
High resolution
A trace amount of a sample can be applicable.
(The lower limit : around 1μg/mL )
A sample containing organic solvents and a suspension sample can be analyzed as well as a waterborne sample.

Example

Quantitative analysis of organic acids in a water solution

《 Result 》
Acrylic acid (AA): 190μg/mL
Methacrylic acid (MA): 60μg/mL

※Sample is measured,
after dilution by 50times with water

Search Number 3022

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